Antifungal and Anti-Biofilm Activities of Acetone Lichen Extracts against Candida albicans.
نویسندگان
چکیده
Candida albicans is a commensal coloniser of the human gastrointestinal tract and an opportunistic pathogen, especially thanks to its capacity to form biofilms. This lifestyle is frequently involved in infections and increases the yeast resistance to antimicrobials and immune defenses. In this context, 38 lichen acetone extracts have been prepared and evaluated for their activity against C. albicans planktonic and sessile cells. Minimum inhibitory concentrations of extracts (MICs) were determined using the broth microdilution method. Anti-biofilm activity was evaluated using tetrazolium salt (XTT) assay as the ability to inhibit the maturation phase (anti-maturation) or to eradicate a preformed 24 h old biofilm (anti-biofilm). While none of the extracts were active against planktonic cells, biofilm maturation was limited by 11 of the tested extracts. Seven extracts displayed both anti-maturation and anti-biofilm activities (half maximal inhibitory concentrations IC50_mat and IC50_biof ≤ 100 µg/mL); Evernia prunastri and Ramalina fastigiata were the most promising lichens (IC50_mat < 4 µg/mL and IC50_biof < 10 µg/mL). Chemical profiles of the active extracts performed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) have been analyzed. Depsides, which were present in large amounts in the most active extracts, could be involved in anti-biofilm activities. This work confirmed that lichens represent a reservoir of compounds with anti-biofilm potential.
منابع مشابه
Evaluation of Anti-Microbial Activity of Elaeocarpus tuberculatus Roxb
In the present study, the antimicrobial efficacy of acetone, methanol and water extracts of leaf, stem bark and fruit of Elaeocarpus tuberculatus Roxb. (Elaeocarpaceae) were examined against four bacterial species (Shigella sonnei, Salmonella typhi, Staphylococcus aureus and Klebsiella pneumoniae) and a fungal species (Candida albicans) using the agar well diffusion method. Results showed that ...
متن کاملThe Evaluation of the virulence factors of clinical Candida isolates and the anti-biofilm activity of Elettaria cardamomum against multi-drug resistant Candida albicans
Background and Purpose: Today, treatment of life-threatening fungal infections, caused by Candida species, has become a major problem. In the present study, we aimed to evaluate the antifungal susceptibility patterns of different clinical Candida isolates, determine the virulence factors in multi-drug resistant (MDR) Candida species, and assess the anti-biofilm activity of Elettaria cardamomum ...
متن کاملAnti-planktonic and anti-biofilm formation activities of the essential oil and hydroalcoholic extract of Myrtus communis L. leaves and fruits against clinical Candida species
Background:Because of the increasing prevalence of fungal infections and antifungal resistance, researchers are seeking new antifungal medications and alternatives. This study aimed to assess the antifungal properties of Myrtus communis L. essential oil and hydroalcoholic extract on Candida species isolated from clinical specimens. Methods: A total of 65 Candida species isolated from clinical ...
متن کاملIn-vitro Antimicrobial and Antitumor Activities of Stevia Rebaudiana (Asteraceae) Leaf Extracts
Purpose: The purpose of the study is to evaluate the antimicrobial and antitumor activities of Stevia rebaudiana (Asteraceae) leaf extracts. Methods: Four solvent extracts (ethyl acetate, acetone, chloroform and water) of Stevia rebaudiana leaves were investigated against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Bacillus subtilis, Aeromonas hydrophila and Vibrio cholerae by us...
متن کاملInhibitory Effect of Fluconazole Combined with Amphotericin B on Fluconazole-Resistant Candida albicans Biofilm Formation
Background & Objective: The incidence of biofilm-related infections caused by Candida albicans has increased dramatically. C. albicans biofilm-related infections are more resistant to antifungal medications. This work was an attempt to examine inhibitory effects of fluconazole in combination with amphotericin B on fluconazole-resistant C. albicans biofilm. Materials & Methods: Fluconazole-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Molecules
دوره 22 4 شماره
صفحات -
تاریخ انتشار 2017